Technical Skills

Proficient in biomaterials and bioengineering research with experience in the design, characterization, and evaluation of novel materials for biomedical applications. Skilled in applying experimental and analytical techniques to study material–biological interactions, tissue engineering approaches, and advanced biomedical device development.

Polymer and Biomaterial Synthesis

Solvent-based anionic and free-radical polymerization of polymeric biomaterials
Cellulose nanofibril isolation and regeneration from cellulose via ionic liquid (IL) treatment
Surface functionalization of biomaterials for bionanotechnology application – NHS-Ester coupling and Click chemistry

Material Characterization

FTIR, 1H-NMR, 13C-NMR, TGA, DSC, SEM, TEM, UV-Vis, XRD, Mastersizer, Rotary Evaporation
Rheological characterization – amplitude sweep test, frequency sweep test, creep-recovery tests

Microfabrication

Microelectromechanical System Technology (MEMS) – mask design, spin coating, sputtering, lift-off lithography, dicing

Cell and Tissue Culture

Mammalian cell (ATDC5, EA.hy926, and AoSMC cell lines) culture, seeding, and splitting
Immune cells (PBMCs, T cells, and NK cells) isolation, culture, and cell banking from whole blood
Organ and tissue sample processing (In vivo) for bioassays and long-term storage

Bioassays and Imaging

Cell Surface Engineering and validation – Ammonia generation assay and Confocal imaging
Cell viability and immune regulation assays – Immunomodulation, Immune cell-mediated cytotoxicity, Complement deposition, Cytokine profiling
Bioassay Tools – Multicolor Flow Cytometry, Microplate Reader, Fluorescence and Confocal Microscopes
Histology data analysis – Masson’s Trichrome, H&E, and C4d staining

Computational and Analytical Tools

Programming and analytical software –MATLAB, R, Origin, GraphPad, MS Office, FlowJo, ChemSketch, Mestrenova, SpinWorks, CytExpert, TopSpin, Adobe Illustrator, BioRender